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    现在位置时时彩网络平台>技术资料首页>产品信息>选购指南>阳离子脂质体试剂的选择

    重庆时时官网开奖结果: 阳离子脂质体试剂的选择

    上海源智生化2010年6月13日 11:10 点击:3007

    时时彩网络平台 www.qlgr.net




    美国英杰Invitrogen 公司  

    当您的实验室开始进行转染时,可以按照下面总结的指南选择试剂。请访问www.invitrogen.com了解加入建议试剂列表的最新资料。

    应 用 建议的试剂(参考文献)
    难于转染的细胞 LIPOFECTAMINE 2000 Reagent (43)
    大部分贴壁细胞 LIPOFECTAMINE 2000 Reagent
    LIPOFECTAMINE PLUS Reagent (17)
    使用血清的贴壁细胞 LIPOFECTAMINE 2000 Reagent (43)
    悬浮细胞 DMRIE-C Reagent (19)
    悬浮CHO细胞 DMRIE-C Reagent (42)
    昆虫细胞 CELLFECTIN Reagent (20)
    内皮细胞 LIPOFECTIN Reagent (21)
    RNA DMRIE-C Reagent (18)
    寡核苷酸 OLIGOFECTAMINE
    主要细胞系进行高效转染建议使用的阳离子脂质体试剂*

    细胞系 哺乳动物细胞来源 建议使用的试剂
    293F Human kidney LIPOFECTAMINE 2000 Reagent
    293H Human kidney LIPOFECTAMINE 2000 Reagent
    BE(2)C Human neuroblastoma LIPOFECTAMINE 2000 Reagent (without serum)
    BHK-21 Hamster kidney LIPOFECTAMINE PLUS Reagent (without serum)
    CHO-K1 Hamster ovary LIPOFECTAMINE 2000 Reagent
    CHO-S (adherent) Hamster ovary LIPOFECTAMINE 2000 Reagent
    CHO-S (suspension)(In CD-CHO Medium) Hamster ovary DMRIE-C Reagent [42]
    LIPOFECTAMINE 2000 Reagent
    COS-1 Monkey kidney LIPOFECTAMINE 2000 Reagent (without serum)
     
    LIPOFECTAMINE PLUS Reagent
    COS7-L Monkey kidney LIPOFECTAMINE 2000 Reagent
    Fibroblasts Human primary passaged LIPOFECTAMINE 2000 Reagent (without serum)
    LIPOFECTAMINE PLUS Reagent (without serum)
    HeLa Human cervical cancer LIPOFECTIN with PLUS Reagent [41]
    HT-29 Human colon cancer LIPOFECTAMINE 2000 Reagent (without serum)
    LIPOFECTAMINE PLUS Reagent (without serum)
    HT-1080 Human fibrosarcoma LIPOFECTAMINE 2000 Reagent (with serum)
    HUAEC (primary) Human endothelial LIPOFECTIN Reagent [21]
    HUVEC (primary) Human endothelial LIPOFECTIN Reagent [21]
    Keratinocytes (In Keratinocyte-SFM) Human primary passaged LIPOFECTAMINE PLUS Reagent [44]
    MDCK Dog kidney LIPOFECTAMINE 2000 Reagent
    MRC-5 Human lung LIPOFECTAMINE 2000 Reagent (without serum)
    LIPOFECTAMINE PLUS Reagent (without serum)
    NIH-3T3 Mouse fibroblasts LIPOFECTAMINE PLUS Reagent
    PC-12 Rat pheochromocytoma LIPOFECTAMINE 2000 Reagent
    SK-BR3 Human breast cancer LIPOFECTAMINE 2000 Reagent (with serum)
    LIPOFECTAMINE PLUS Reagent (without serum)
    Vero Monkey kidney LIPOFECTAMINE 2000 Reagent (without serum)

    细胞系 昆虫细胞来源 建议的试剂
    D.Mel-2 r Drosophila melanogaste CELLFECTIN Reagent
    Sf9 Spodoptera frugiperda CELLFECTIN Reagent
    Sf21 Spodoptera frugiperda CELLFECTIN Reagent
    *这些转染步骤可以在www.invitrogen.com的Technical Resource部分下载。

    其他使用阳离子脂质体成功转染的细胞

    Invitrogen已经总结了800多篇使用我们的阳离子脂质体试剂成功细胞的参考文献。下面的小数据库可以在我们的网站上获取。在以上表格中没有列出的细胞类型和阳离子脂质体类别总结在下面的表中。

    哺乳动物细胞类型 来 源 细胞系 试 剂
    Adipocytes Mouse 3T3-F442A LIPOFECTAMINE Reagent
    Adrenal Human carcinoma SW13 LIPOFECTIN Reagent
    Amniocytes Human Primary LIPOFECTIN Reagent
    Bladder Human carcinoma T24 LIPOFECTIN Reagent
    Bone Human sarcoma U-2 OS LIPOFECTIN Reagent
    Bone marrow Human leukemia KG-1; KG1a; K562 DMRIE-C Reagent
    Breast Cancer Human MCF7
    Hs 578T; T-47D;
    MDA-MB-231; ZR-75-1
    LIPOFECTAMINE PLUS Reagent
    LIPOFECTAMINE Reagent
    Mouse
    Rat carcinoma
    C127 NMU LIPOFECTIN Reagent
    LIPOFECTAMINE Reagent
    Cervical Cancer Human C-33 A LIPOFECTAMINE Reagent
    Colon Human cancer HCT116
    COLO 320 HSR; SW403
    SW480; Caco-2
    LIPOFECTAMINE PLUS Reagent
    LIPOFECTAMINE Reagent
    Human cancer T84 LIPOFECTIN Reagent
    Connective Tissue Mouse L LIPOFECTAMINE Reagent;
    LIPOFECTIN Reagent
    Endometrial Cells Human cancer Isikawa LIPOFECTAMINE Reagent
    Endothelial Rabbit Primary LIPOFECTIN Reagent
    Embryonic carcinoma Mouse P19; F9 LIPOFECTAMINE PLUS Reagent
    Fibroblasts Mink lung
    Mouse
    Mv 1 Lu
    TA1; Ψ-2;
    NIH 3T6; 10T 1/2
    LIPOFECTIN Reagent
    LIPOFECTAMINE PLUS Reagent; or
    LIPOFECTAMINE Reagent;
    orLIPOFECTIN Reagent
    Kidney Cat
    Human carcinoma
    Monkey
    Pig
    Sheep

    A498; UMRC 6
    CV-1
    LLC-PK
    FLK
    LIPOFECTIN Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent
    Liver Dog
    Human hepatoma
    Mouse
    Rat hepatocytes
    Rat hepatoma
    Primary
    Huh7
    Hep G2
    Primary
    Primary
    FTO-2B
    MSN610.2;Morris 7777;
    H35; McA-RH7777
    LIPOFECTIN Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE PLUS Reagent
    LIPOFECTIN Reagent
    LIPOFECTIN Reagent
    LIPOFECTAMINE PLUS Reagent
    LIPOFECTAMINE Reagent; or
    LIPOFECTIN Reagent
    Lung Hamster

    Human
    Human cancer Human carcinoma
    Human small cell carcinoma
    R1610
    Dede
    WI-38
    NCI-H23
    A549
    H187; NCI-H146
    LIPOFECTAMINE PLUS Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTIN Reagent
    LIPOFECTIN Reagent
    Lymphoid Bovine leukemia
    Human lymphoma

    Human leukemia
    Mouse B cell lymphoma Mouse lymphoma
    BL3.1
    Jurkat
    U937
    MOLT-4
    A20.J; M12
    P388D1
    LIPOFECTAMINE Reagent
    DMRIE-C Reagent
    LIPOFECTIN Reagent
    DMRIE-C Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent
    Macrophages/monocytes Human monocyte
    Mouse macrophage
    THP-1
    Primary; J774.16
    RAW 264.7
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent; or
    LIPOFECTIN Reagent
    Melanoma Mouse B16 LIPOFECTAMINE Reagent
    Muscle Cow
    Mouse myoblast

    Rat
    Rat myoblast
    Rat smooth muscle
    Trachea smooth muscle
    C2C12
    Primary; G8
    Primary
    L6E9
    A10
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE PLUS Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTIN Reagent
    LIPOFECTIN Reagent
    LIPOFECTAMINE Reagent
    Neuronal cells Human glioma
    Human neuroblastoma
    Human neuroblastoma
    Mouse neuroblastoma Rat astrocytes
    Rat glioma
    U-87; A-172
    SK-N-MC
    SK-N-SH
    Neuro-2a
    Primary fetal
    C6
    LIPOFECTIN Reagent
    LIPOFECTIN Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent
    Ovary Hamster Reagent

    Human cancer
    Mouse
    CT60; AS52; CHO-DXB11

    SKOV3
    Primary
    LIPOFECTAMINE PLUS Reagent; or
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTIN Reagent
    Pancreas Human carcinoma
    Rat
    Rat islet cells
    Capan-2
    AR4-2J
    Primary fetal
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTIN Reagent
    Pituitary Mouse
    Rat
    Rat tumor
    AtT-20
    Primary
    GH3
    LIPOFECTAMINE Reagent
    LIPOFECTIN Reagent
    LIPOFECTAMINE Reagent
    Prostate Human LNCaP LIPOFECTIN Reagent
    Skin Human Primary keratinocytes LIPOFECTAMINE PLUS Reagent
    Spleen Mouse AKR LIPOFECTIN Reagent
    Sperm Mouse   LIPOFECTIN Reagent
    Stem cells Human CD34+
    Mouse
    Primary hematopoetic
    D3
    DMRIE-C Reagent
    LIPOFECTIN Reagent
    Thyroidt Rat FRTL-5 LIPOFECTAMINE PLUS Reagent
    Uterus Human SK-UT-1 LIPOFECTAMINE Reagent

    其他细胞类型 来 源 细胞系 建议试剂
    Chicken   Blastoderm cells
    Primary chondrocytes
    Primary fibroblasts
    LIPOFECTIN Reagent
    LIPOFECTAMINE Reagent
    LIPOFECTAMINE Reagent;
    LIPOFECTIN Reagent
    Corn   BMS-M protoplasts LIPOFECTIN Reagent
    Dictyostelium     LIPOFECTIN Reagent
    Frog Xenopus laevis A6 kidney cells Eggs;
    kidney cells
    LIPOFECTAMINE PLUS Reagent
    LIPOFECTIN Reagent
    Insect Drosophila
    Spodoptera frugiperda
    D.Mel-2; Schneider’s L2
    Sf9; Sf21
    CELLFECTIN Reagent
    CELLFECTIN Reagent
    Neurospora crassa     LIPOFECTIN Reagent
    Rice   DGZ protoplasts LIPOFECTIN Reagent
    Shrimp   Primary lymphoid LIPOFECTIN Reagent
    Tobacco   Protoplasts LIPOFECTIN Reagent
    Yeast Saccharomyces cerevisiae
    Schizosaccharomyces pombe
      LIPOFECTIN Reagent
    LIPOFECTIN Reagent

    (来源: 上海源智生化 )


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